Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

quattromicro API

http://www.chromforum.org/viewtopic.php?t=26952

Page 1 of 1

quattromicro API

Posted: Thu Jun 11, 2015 9:01 pm
by abdulsar
Hi All,

Wondering if anyone has experienced similar problem.
Recently I have been having a problem with the signal stability in Quattro Micro API, I am using an ESI probe and it was working fine. Suddenly while I was trying to run direct infusion, the signal seems redundant and it keeps fluctuating badly.
I have tried to change the source parts but it is not working.

Any idea where the problem might be?

Thanks

Re: quattromicro API

Posted: Wed Jun 17, 2015 5:16 am
by dr_bahram1977
Please let us know if you've baseline problem, or this is about your peaks.

Re: quattromicro API

Posted: Thu Jun 18, 2015 10:26 pm
by abdulsar
Please let us know if you've baseline problem, or this is about your peaks.
The analyte peak appears and disappears when I am operating at low flow rate 10 ul/min, the baseline seems fine however!

Re: quattromicro API

Posted: Sun Jul 05, 2015 12:22 pm
by Thys
Main reason for an unstable signal in infusion is the capilairy. Try to rebuild the probe and check the grafite ferrule. Maybe this one is set to loose or maybe the ferrule is damaged by overtighting. Also deadvolume on this connection might influence the signal.
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/