Chromatography Forum

Hi,

I am running a method using a polyfluorophenyl LC Column. I am analyzing Taxol samples prepared in Sodium Dodecyl Sulfate in pH 4.0 buffer solution. I am having continuous issues with inconsistent retention time and peak response reproducibility for the taxol peak using multiple columns of the same type and 57:43 Water:ACN as mobile phase. I've tried washing the column with a 50:50 Methanol Water mix but the issues keep returning. Has anyone experience using SDS on a Phenyl column, and knows of any issues there may be when using this combination?
Thanks for any advice.

Check out this source: www.chem.agilent.com/Library/.../5988-7973EN.p...
Or this source: https://www.phenomenex.com/.../HPLCDetail/Curosil
Or this source: Chromegabond™ PFP/T HPLC Columns - ES Industries
Or this source: www.sigmaaldrich.com/catalog/product/.../g005890
and many others. SDS should not make any trouble on a Phenyl column. Look for the applications to see what they did.
At what temperature you are running your method? Did you check your flow rate at each channel?
Good luck

Check out this source: http://www.chem.agilent.com/Library/.../5988-7973EN.p...
Or this source: https://www.phenomenex.com/.../HPLCDetail/Curosil
Or this source: Chromegabond™ PFP/T HPLC Columns - ES Industries
Or this source: http://www.sigmaaldrich.com/catalog/product/.../g005890
and many others. SDS should not make any trouble on a Phenyl column. Look for the applications to see what they did.
At what temperature you are running your method? Did you check your flow rate at each channel?
Good luck

Thanks for the links but they seem to be down.
Sample temp is 23 C. Column heater 50 C. Flow rate is monitored and is seen to be constant at 1.5ml/min.

http://derpharmachemica.com/vol2-iss2/D ... 09-115.pdf
Hope that link will work.
So far I remember such problems as monitored by you lead in the past to special Taxol columns from different manufacturers.
Maybe 50°C for the column is a little bit too high, or better to say the temperature difference between sample and column too high in my opinion.
Column equilibration seems to be critical with these Taxol methods.