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Question about C-18 extraction disks

Posted: Tue Jun 02, 2015 8:04 pm
by Mike H.
I have just compared the system blank chromatograms for a gradient run where the mobile phase was filtered through a C-18 extraction disk and one where it was not. The blank for the filtered run is noticeably cleaner than the non-filtered blank. Why? I don't understand why mobile phase impurities would be detected as peaks when it seems like they would be masked by the background.

Re: Question about C-18 extraction disks

Posted: Tue Jun 02, 2015 8:08 pm
by tom jupille
Explanation is here: viewtopic.php?f=31&t=19085

Re: Question about C-18 extraction disks

Posted: Wed Jun 03, 2015 12:59 pm
by Mike H.
I had forgotten how well you are able to explain these concepts. (I took your method development online course a couple years ago.) I now think I should have started with the troubleshooting course first. Thank you.