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Pentafluorinated phenyl column / quarternary amines

http://www.chromforum.org/viewtopic.php?t=2690

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Pentafluorinated phenyl column / quarternary amines

Posted: Wed Oct 05, 2005 6:41 pm
by LCFlo
Hi

What is a possible mobile phase for quarternary amines when using a pentafluorinated phenyl column ?

Thank you for every suggestion ?

Florian

PS: 100% Acn (30 Minutes): no peak

Posted: Thu Oct 06, 2005 2:10 am
by SIELC_Tech
Flo,

Why are you using 100% ACN. Residual silanol will act as weak acidic ion exchange and will hold you strong amine. Try to add water and a little of acid (TFA, sulfuric or any buffer). You might need very little amount of ions in the mobile phase.

Also inject sample without the column and measure peak area. May be something is wrong with your injection/ detection.

Are you separating dequalinium chloride? (You did it while ago).

Regards,

Vlad
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