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- Posts: 1
- Joined: Thu Sep 29, 2005 10:16 pm
I'm using a GPC column with a pH 7,0 mobile phase (0,4M phosphate buffer) to detect and assay hyaluronic acid (MW:10xE6 D) in pharmaceutical products. The method I validated had plate count of 2000. Plate count values are reccomended higher than 4000, but I think it is not possible to increase plate count with this tecnique, as retention is only physical.
Am I wrong?
Thanks in advance!
A.M.