Internal Standard for Palm FAME
Posted: Sun Oct 02, 2005 1:42 pm
Hello everyone.
My company is currently facing some problem with different content (percentage differ up to 2 units) of C18:2 and C:16 with outside laboratory GLC result.
My questions are:-
1) May I know how to prepare our Internal Standard for palm oil fatty acid methyl ester?
2) Do I need to use External Standard to prepare (quantify) my Internal Standard?
Our GC FAME is using fast sample preparation method, which we insert 3 drop of oil into the test tube, add 1.4ml of N-Heptane, then add up 3 drops of sodium methanolic solution, and then vortex. Then add up some sulphate powder and vortex and finally filter into vial.
My company is currently facing some problem with different content (percentage differ up to 2 units) of C18:2 and C:16 with outside laboratory GLC result.
My questions are:-
1) May I know how to prepare our Internal Standard for palm oil fatty acid methyl ester?
2) Do I need to use External Standard to prepare (quantify) my Internal Standard?
Our GC FAME is using fast sample preparation method, which we insert 3 drop of oil into the test tube, add 1.4ml of N-Heptane, then add up 3 drops of sodium methanolic solution, and then vortex. Then add up some sulphate powder and vortex and finally filter into vial.