Effectiveness of Acetic Acid for Ion Pairing
Posted: Wed Apr 15, 2015 4:53 pm
Hey All
I want to ask about the effectiveness of Acetic Acid as an ion pairing agent: for proteins or for small molecules with a positive charge.
My sense is that acetic acid (or to be more precise, the acetate ion) does pair, though not as well as something like TFA. And, as a result, one needs to use a higher concentration. But that ultimately it will work, just at a higher concentration.
Please let me know what your experience has been. And what concentration levels have you found to be necessary.
Thank You
I want to ask about the effectiveness of Acetic Acid as an ion pairing agent: for proteins or for small molecules with a positive charge.
My sense is that acetic acid (or to be more precise, the acetate ion) does pair, though not as well as something like TFA. And, as a result, one needs to use a higher concentration. But that ultimately it will work, just at a higher concentration.
Please let me know what your experience has been. And what concentration levels have you found to be necessary.
Thank You