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Detector out resistance effect on peak width.

Posted: Thu Apr 09, 2015 12:21 pm
by Johte
Good Morning,

I was told I could decrease my peak width in a nanoflow instrument by increasing the resistance of flow ( with length of silica line ) of detector out that I can prevent diffusion of my eluted bands. If someone could link me to some literature on the subject it would be most helpful.

Thank you.

Re: Detector out resistance effect on peak width.

Posted: Thu Apr 09, 2015 12:33 pm
by kubowicz.tomasz
Hello

Be careful with installing any restriction in detector outlet - you can damage detector cell if pressure will be to high (check limit for cell).

Regards

Tom