Linearity Curve starting from zero

[jayroseville58]

Is it allowable for us to perform linearity test on our method validation Starting sample with unspiked standard solution? Thanks.

[Fernando]

Hi jayroseville58

If you are talking of an assay you have to perform the linearity at 80 % ; 90 %; 100 %; 110 % and 120 % of the concentration you begin working when you develop the method.
In case you have to work near zero I think you have to find first the limit of quantitation (LOQ).

Best regards

Fernando

[DR]

The merits and hazards of forcing your curve through zero have been discussed several times on this forum - use the search if you need more info.