Does anyone have experience on HPLC analysis of MPL? I found a couple of methods online and neither worked for me. I tried water/ACN. water/Methanol, and water/IPA as mobile phase, a C8 column, and CAD detection. The problem was that no peak was observed for MPL unless I use high organic isocratic mobile phase, such as 100% ACN, in which case, MPL elutes at void volume. Any help is appreciated.