Very annoying background noise
Posted: Wed Mar 25, 2015 1:06 pm
Hi all,
I have started using LCMS-QTOF Brukerfor three months and we have a problem on the instrument background in positive mode. there are two masses of 86 and 114 at 10*4 magnitude that are persistently present in the system using a HILIC column. We use ACN in B and water(MS grade) in C with formic acid 1% on both solutions and also 10mM Ammonium formate in solvent A. I have been washing the entire system with those solutions but the problem still persists. We also use a ramping approach for the solvents A and B.
Do you have any comments and ideas of how to get rid of these stubborn compounds?
Thanks for your help
I have started using LCMS-QTOF Brukerfor three months and we have a problem on the instrument background in positive mode. there are two masses of 86 and 114 at 10*4 magnitude that are persistently present in the system using a HILIC column. We use ACN in B and water(MS grade) in C with formic acid 1% on both solutions and also 10mM Ammonium formate in solvent A. I have been washing the entire system with those solutions but the problem still persists. We also use a ramping approach for the solvents A and B.
Do you have any comments and ideas of how to get rid of these stubborn compounds?
Thanks for your help
