trouble with degassed UPLC Water

[ndtrung0807]

When i load my method to run baseline, i showed delta pressure <20 but later the screen notify Degassed failed and the machine is stopped pump.
anybody know this problem?
Please help me solve it
Thank you

[lmh]

This is probably a broken degasser. Many degassers work by splitting the liquid flow into a lot of thin-walled tubes that pass through a vacuum chamber, so that gasses in the liquid can diffuse out through the thin walls and be pumped away. Sometimes the walls fail, and liquid gets out into the vacuum chamber. Sometimes the little vacuum pumps fail. If liquid gets out into the chamber, it almost always damages the little vacuum pump. Very often degasser failures are a matter of replacing the chamber and/or pump, which is probably a job for the manufacturer. If you are trying to work out which bit has gone wrong yourself, it can be useful to find the outlet tube for the vacuum pump and see whether liquid has got into it. This will confirm that at least one channel of the degasser has got broken pipe-work and needs replacement.

In the short term, if your method is not very sensitive to dissolved gas, you may be able to deconfigure the degasser and turn it off, so that the software will run without creating an error because the degasser is unable to work properly. Ideally you will want to bypass any channels of the degasser that may be leaking. You can use manually degassed solvents.

[ndtrung0807]

This is probably a broken degasser. Many degassers work by splitting the liquid flow into a lot of thin-walled tubes that pass through a vacuum chamber, so that gasses in the liquid can diffuse out through the thin walls and be pumped away. Sometimes the walls fail, and liquid gets out into the vacuum chamber. Sometimes the little vacuum pumps fail. If liquid gets out into the chamber, it almost always damages the little vacuum pump. Very often degasser failures are a matter of replacing the chamber and/or pump, which is probably a job for the manufacturer. If you are trying to work out which bit has gone wrong yourself, it can be useful to find the outlet tube for the vacuum pump and see whether liquid has got into it. This will confirm that at least one channel of the degasser has got broken pipe-work and needs replacement.

In the short term, if your method is not very sensitive to dissolved gas, you may be able to deconfigure the degasser and turn it off, so that the software will run without creating an error because the degasser is unable to work properly. Ideally you will want to bypass any channels of the degasser that may be leaking. You can use manually degassed solvents.

Thank you lmh!
I removed check valve device and take filter device to ultrasonic by acid HNO3 1%,methanol 1% about 30min, then i installed again and restarted pump, everything is ok, but several day later, this above situation happend again.
according to you, if chamber or pump was error and should replace, how much does it cost?
Thank you

[bundy5555]

Your degassing chamber or the tubing need to be serviced or changed.

It is unlikely that your vacuum pump is broken since it is designed to last for 10+ years under normal operating condition. Even in case of leaking, the instrument will automatically shut pump off to protect the pump.

My suggesting is to change your vacuum chambers and connection tubing every 12 months if you are running on normal phase and every three years on reverse phase.

For the temporary fix, tighten the nuts and screws on the outside and inside of the vacuum chambers. Check you vacuum tubing, organic solvent may cause the soft tubing to expand and cause lost of vaccum.

For replacement cost, it will cost you $2000+USD for part and labour to replace 4 chambers and vacuum line if you want call for certified technician. However, if you know how to DIY, the parts only cost about $500 if I remembered correctly. It is really easy and only takes about 1hour to do it.

[ndtrung0807]

Your degassing chamber or the tubing need to be serviced or changed.

It is unlikely that your vacuum pump is broken since it is designed to last for 10+ years under normal operating condition. Even in case of leaking, the instrument will automatically shut pump off to protect the pump.

My suggesting is to change your vacuum chambers and connection tubing every 12 months if you are running on normal phase and every three years on reverse phase.

For the temporary fix, tighten the nuts and screws on the outside and inside of the vacuum chambers. Check you vacuum tubing, organic solvent may cause the soft tubing to expand and cause lost of vaccum.

For replacement cost, it will cost you $2000+USD for part and labour to replace 4 chambers and vacuum line if you want call for certified technician. However, if you know how to DIY, the parts only cost about $500 if I remembered correctly. It is really easy and only takes about 1hour to do it.

Thank you bundy5555
I think that's explain correctly!
Thank for your advice

Tom

[danko]

I don't care much about degassers.
Think they're pain in the neck. And provide falls peace of mind.
Just toss the degasser in the bin and degass your mobile phase/s within use and you'll be fine - always.

Best Regards