Agilent 1260

[Besma2]

WANT TO SCANNING A CAFFEINE SOLUTION WITH HPLC Agilent 1260 FOR CHECKING THE WAVE LENGTH, CAN YOU HELP ME PLEASE? HOW DO I PROGRAM MY METHOD (PART DETECTOR)
THANKS

[AnalyticalChem]

Caffeine absorbs in the spectral range between 244 nm to 300 nm
in dichloromethane and water with λ max at 276 nm and 272.8 nm respectively, 250 nm to 300 nm at
λ max of 276 nm in chloroform and 258 nm to 300 nm at λ max of 274.4nm in ethyl acetate.

[kubowicz.tomasz]

Hello

DAD: inject caffeine and if you have spectra option installed you can collect full spectrum
VWD: fill cell with caffeine and take sample scan (you can use chemstation or hand controller)

Good luck

Tomasz Kubowicz

[Besma2]

Hello
Tomasz Kubowicz

thank you very much
I want to use ChemStation , you can please describe me the steps to create the scanning method and how to recover my results .
eg for the detector I have to select what
and once in the offline I check how the spectrum
thank you again