purification of modified synthetic oligonucleotides w/ HPLC
Posted: Mon Feb 23, 2015 1:10 pm
Hello experts,
I'm a student from Ege University, Turkey.
I am working on a project and production of q-PCR probes which are labeled with other molecules. Here are some example of them;
FL Probe; CTATTGGTTTGGCAATcTGAT-FL-3’
LC Probe; 5’-LC-640-TTCTGTGAGCCCAGTAGCAGCTATAGTAACT-Ph-3’
http://www.fluoresentric.com/documents/HybProbe.pdf First page of this document represents some information about chemical structure of probes.
Synthesis process is going well i think. I want to separate them from successful products.
I have used a gradient method with a PLRP-S column (particle diameter of 20 microns) but it's not worked well. My lc labes were hold in the column.
What kind of column and method do I need to use?
Thank you for your help.
Best regards.
I'm a student from Ege University, Turkey.
I am working on a project and production of q-PCR probes which are labeled with other molecules. Here are some example of them;
FL Probe; CTATTGGTTTGGCAATcTGAT-FL-3’
LC Probe; 5’-LC-640-TTCTGTGAGCCCAGTAGCAGCTATAGTAACT-Ph-3’
http://www.fluoresentric.com/documents/HybProbe.pdf First page of this document represents some information about chemical structure of probes.
Synthesis process is going well i think. I want to separate them from successful products.
I have used a gradient method with a PLRP-S column (particle diameter of 20 microns) but it's not worked well. My lc labes were hold in the column.
What kind of column and method do I need to use?
Thank you for your help.
Best regards.