Chromatography Forum

Seeking verification that Empower 3 requires that the syringe volume be specified (10 uL syringe and I know this is the case) and that the nanoliter adapter option must be selected (checked) in order to ensure I get a 1 uL injection.

We just upgraded to Empower 3. Previously, with Empower 2, we left the nanoliter box unchecked to ensure a 1 uL injection as specified in the Empower 2 sample set.

I just investigated this on a 6890N system, and I clearly see that if that option is not selected (if the nanoliter adapter box is unchecked) then I only get a 0.2 uL injection. However, if I check off that box (knowing all of our GCs have the NL adapters) and I further specify a 10 uL syringe (as has always beeen the case) then I do get the 1.00 uL injection as specified in my Empower sample set method.

I just wanted to get some kind of confirmation regarding this setting.

The 6890s here in house both require nanoliter adapter and specified syringe volume to be ticked to ensure that the correct volume is injected. This was the same for us across Empower 2 and 3. Has your nanoliter adapter been added in a recent service, or has your injector tower model changed since your upgrade from Empower 2 to Empower 3?

"The injection volume displayed in Run Samples can be expressed as an actual volume or
as the number of plunger steps injected by the GC.
You can specify the syringe size and presence of the nanoliter adapter for the front and
back injectors. When you set these parameters, Empower software calculates the actual
injection volume. Otherwise, the injection volume shown in the Run Samples window is
expressed as a raw value of the number of plunger steps injected."

Taken from the Empower 2 GC manual.