PROTEIN--AAA -HPLC-UV,OR LCMS,LCMSMS

[wader]

Is anybody did the test of amino acids for protein only one run for all AAA?



Thanks

[yazmer]

Yes, actually if you want to do this by HPLC, you will usually need to derivatize and use a fluorescence detector. However, if you have an MS, things get easier. Although I hear now you don't have to derivatize anymore

I know shimadzu has a system dedicated to this:

http://www.shimadzu.com/an/hplc/aplsys/uf-amino.html

And i'm confident if you have a triple quad, many other vendors like sciex, etc will all have solutions.