UV detection
Posted: Fri Jan 16, 2015 3:39 pm
do you guys see larger variation on one wavelength, but smaller variation on another wavelength. Both wavelength has very good S/N. Larger than 50.
Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/
Certainly. Especislly if one of the wvelengths is at the top of a broad absorbance band and the other is on a steep slope.
Usually, yes. Exceptions might be if a different wavelength avoids a close-eluting interference, if the baseline is especially noisy at the absorbance maximum, or if there are multiple analytes with different UV spectra and you want to avoid switching wavelengths.