UV detection

[jiangds06]

do you guys see larger variation on one wavelength, but smaller variation on another wavelength. Both wavelength has very good S/N. Larger than 50.

[tom jupille]

Certainly. Especislly if one of the wvelengths is at the top of a broad absorbance band and the other is on a steep slope.

[jiangds06]

so the UV maxima should be the wavelength of choice.

Certainly. Especislly if one of the wvelengths is at the top of a broad absorbance band and the other is on a steep slope.

[tom jupille]

Usually, yes. Exceptions might be if a different wavelength avoids a close-eluting interference, if the baseline is especially noisy at the absorbance maximum, or if there are multiple analytes with different UV spectra and you want to avoid switching wavelengths.

[jiangds06]

Thanks.

Usually, yes. Exceptions might be if a different wavelength avoids a close-eluting interference, if the baseline is especially noisy at the absorbance maximum, or if there are multiple analytes with different UV spectra and you want to avoid switching wavelengths.