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peak area consistently decrease
Posted: Thu Jan 08, 2015 4:56 pm
by jiangds06
1424616
1423318
1202280
1133670
1137694
1202050
do you know what is the problem, retention time is fixed.
Re: peak area consistently decrease
Posted: Fri Jan 09, 2015 6:51 am
by Gerhard Kratz
Please, we need more details. My crystal ball is frozen. What sample compound, what column (C18 is not enough), mobile phase etc.?
Could be that your compound is disappearing from the vial, absorption to glass or the stationary phase. Many options.
Re: peak area consistently decrease
Posted: Fri Jan 09, 2015 6:54 pm
by uzman
Actually areas are consistent by pairs. ( 1424616 - 1423318 - 1202280 -1202050 -1133670 -1137694 )
It looks like there is a problem on autosampler .
Re: peak area consistently decrease
Posted: Mon Jan 12, 2015 6:37 pm
by jiangds06
Yes, there two additional samples, which are inserted in between every other two samples.
Actually areas are consistent by pairs. ( 1424616 - 1423318 - 1202280 -1202050 -1133670 -1137694 )
It looks like there is a problem on autosampler .