Dear all, I have a question. Are the requirements of Commission Decision 2002/657/EC suitable in the analysis of biological samples in conducting of bioequivalence studies? We have triplequad MS in our lab and usually work in mrm-mode. Usually we detect only one MRM transaction (the most intense) for each compound. I think it is really not very good approach, because we have "quantifier" fragment of parent-ion, but don't have any "qualifiers". Maybe somebody works with the same samples using triple quad MS-MS? How many ion-transaction's do you register for reliable identification and quantification? Do you use maximum permitted tolerances for relative ion intensities, mentioned in Commission Decision 2002/657/EC?

PS - here you can read text of Commission Decision 2002/657/EC http://eurl.craw.eu/img/page/Legislatio ... 002_EN.pdf