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- Posts: 85
- Joined: Wed Oct 19, 2011 9:46 am
Hi guys.
We have a method in our lab to determine the content of folic acid by hplc in tablets, which also have iron glycinate. Column C18 (Gemini), MP Potassium phosphate buffer (2 g/L) 650mL + tetrabutylammonium hydroxide methanolic1M 12 mL + H3PO4 3N 7.0 mL + Methanol 250mL pH adjusted to 7.0 and diluted to 1000 mL with deion. water, isocratic method. But after some injections, the corresponding peak starts to deform at the baseline. I have assumed that the Iron is accumulating at the head of the column, so I washed it with 0.5 % H3PO4 inverted flow. It worked, but I want your opinion if I did right, because if that´s the problem, I can convince my boss to buy me a pre-column. Or are there any other cheap solution? I don´t want to change my method and washing it inverted flow doesn`t make me happy either.
We have a method in our lab to determine the content of folic acid by hplc in tablets, which also have iron glycinate. Column C18 (Gemini), MP Potassium phosphate buffer (2 g/L) 650mL + tetrabutylammonium hydroxide methanolic1M 12 mL + H3PO4 3N 7.0 mL + Methanol 250mL pH adjusted to 7.0 and diluted to 1000 mL with deion. water, isocratic method. But after some injections, the corresponding peak starts to deform at the baseline. I have assumed that the Iron is accumulating at the head of the column, so I washed it with 0.5 % H3PO4 inverted flow. It worked, but I want your opinion if I did right, because if that´s the problem, I can convince my boss to buy me a pre-column. Or are there any other cheap solution? I don´t want to change my method and washing it inverted flow doesn`t make me happy either.
Q. F. Ignacio Viera
