UV method Help
Posted: Tue Dec 09, 2014 6:36 am
Kindly share the UV method of Vitamin A determination in food
Instrument parameters
Temperature 40 °C
Injection amount 5 μL
Detection UV (DAD), 325 nm for retinyl palmitate, 460 nm for beta carotene
Flow rate 1.0 mL/minute
Mobile phase 70/30 methanol with 3.2 g/L ammonium acetate/acetonitrile
(isocratic, premixed)
C18, 4.6 × 150 mm, 3.0 μm column