Hello guys,
The sentence from "Column user guide for Agilent Normal-Phase and HILIC Columns" :
"The RRHD Hilic column cannot be backflushed."
Why is that ? They mount 0.5μm column inlet frit...so whats the problem with backflushing ?
Any other reason ?
Sometimes, during the column packing process the pore size of the outlet frit is actually higher than the particle size. I have never seen particles flushing out of the frit. I feel this is an unfounded fear that the particles will flow out of the frit if the pore size is similar as the particles. This apparently never happens. I may be wrong.