Advice on quantification with hexadecane
Posted: Sat Nov 29, 2014 11:11 pm
by AA11
Currently, I'm using a GC-MS with a HP-5 column to quantify phenol and cyclohexanol which does a good job with quantification in methylene chloride solvent by itself. I am running reactions in hexadecane which is not a good solvent for phenol or cyclohexanol and am using toluene as a co-solvent, but I am having issues with injection repeatability on 75% of my samples. The liner has been changed out and so has the inlet septum. The column is free of obstruction. I have tried to run at a higher inlet temp (310 deg C), but the signal of the low boiling pt components is bad. Is there a way I can get good quantification of phenol and cyclohexanol using the GC? Will I have to use a solvent that is immiscible in hexdecane for quantification? Would LC be a better choice?
Thanks for any suggestions.
Re: Advice on quantification with hexadecane
Posted: Mon Dec 01, 2014 3:38 pm
by Consumer Products Guy
Would LC be a better choice?
No, I think GC is a better choice. If you "must" have hexadecane in your matrix, if me I'd try diluting/disssolving all in DMF, keep your column type as it is.
We've used hexadecane successfully as solvent when assaying high-boiling paraffins.
Re: Advice on quantification with hexadecane
Posted: Mon Dec 01, 2014 4:03 pm
by AA11
Would LC be a better choice?
No, I think GC is a better choice. If you "must" have hexadecane in your matrix, if me I'd try diluting/disssolving all in DMF, keep your column type as it is.
We've used hexadecane successfully as solvent when assaying high-boiling paraffins.
So you are inferring that Hexadecane will cause issue with quantification?
Re: Advice on quantification with hexadecane
Posted: Mon Dec 01, 2014 5:14 pm
by Consumer Products Guy
So you are inferring that Hexadecane will cause issue with quantification?
No.