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UV Detector Calibration

Posted: Mon Nov 24, 2014 10:07 am
by StephenO
Is there any guideance regarding how often you should change the lamp in a UV detector?
Should it be stated in your Procedures how often you repalce the lamp?

Thanks in advance

Re: UV Detector Calibration

Posted: Mon Nov 24, 2014 11:22 am
by Gerhard Kratz
Maybe the software will tell you to change the lamp, or you will find some information in the manual how to control the lamp.
Manufacturer can tell you the average life time of the lamp.

Re: UV Detector Calibration

Posted: Mon Nov 24, 2014 12:49 pm
by Kreall
The software should tell you that you should change your UV lamp via intensity of lamp. But bottom line you use lamp until it breaks down.

Re: UV Detector Calibration

Posted: Mon Nov 24, 2014 3:23 pm
by HPLCaddict
The software should tell you that you should change your UV lamp via intensity of lamp. But bottom line you use lamp until it breaks down.
Objection: For Pharma the Bottom line is that you use the lamp as long as it's appropriate for the specific analysis. An old lamp might lead to a significant higher LOQ of your methods. Related substances, trace analysis and similar therefore should include some sort of System suitability check (e.g. signal/noise of a low-concentrated sample in the specification region) to verify the lamp energy is still high enough. For other, high-concentrated stuff I'd follow your suggestion - use it until it breaks.

Re: UV Detector Calibration

Posted: Mon Nov 24, 2014 3:55 pm
by StephenO
Thanks for all the advice.