Chromatography Forum

Hi everybody in this forum,
I need to remove Fe3+ from a solution and I was given the following resin http://www.bio-rad.com/en-us/product/ma ... ange-resin
I guess that according to resin properties (sulfonic acid group, 25 um particle size) it should be a good choice for binding Fe in the solution. I not really sure why but Fe is not binding to the resin because Fe titration before and after resin binding in batch purification (not column) is giving the same Fe concentration.
After this results I turned to the following Biorad Manual for Ion contaminant removal http://www.bio-rad.com/LifeScience/pdf/ ... n_1825.pdf
and it suggests the AGW 50 resin for heavy metal removal. However, the caracteristicis of the resin as to particle size and functional groups are almost the same.

May you help me with this? Why won´t I be able to use the Macro Prep Resin? Any recommended protocol?

Thank a lots,

Hi

You can use the so called "On Guard II Catridges" from ThermoFisher. These cartridges are often used in ion chromatography sample pretreatment and available in different sizes (1ml/ 2.5ml) and different chemistries. For details see the linked manual (http://www.dionex.com/en-us/webdocs/436 ... _FINAL.pdf. The most interesting part for you is on page 27-29. More info also could be found here: http://www.dionex.com/en-us/products/ac ... 73683.html

I wish you success!

Best

Thank you Stefan for all of the information.
However, I was wondering about the difference between Biroad resins and why the MacroPrep 25S cannot be used for Fe remotion even when it is very similar to the AG50W which is recommended by Biorad.

I appreciate it if you can help with that,

Thank you,

Those two resins are about as similar as an elephant and a mouse (both are warm-blooded, have hair, give birth to live young, and are colored gray, but they have different dietary requirements, and I certainly would not want to ride a mouse on a safari).

The resins are both polystyrene-divinylbenzene sulfonates, but they have different crosslinking, different specific surfaces, vastly different pore structures, and different ion-exchange capacities.