hi everybody,
i have been doing rs analysis for many finished as well as api. the method is usually from the api vendor. most of the time the concentration of the sample is like 10mg/ml.usually i calculate by area normalisation. i would like to know
1) how do they decide the sample concentration
2)can i used the assay std if area normalisation is appliedcoz we use it for rt checking
3)which ich guide line no. explains full details about it

if anybody help me out regarding my doubt i will be very thankful to all.
have a nice day to all.
with regard
zydus