-
- Posts: 36
- Joined: Tue Jun 10, 2008 6:49 pm
Hi,
Background: I am working on a Glucose and Fructose derivative separation. We purchased a Shodex Asahipak NH2P-50 based on a few papers that use the column. We are getting some separation, but it is not baseline resolved (MS detection). I noted in the Shodex literature that their Pb+ column may be better suited. Our current setup is with an LC-DAD-MS and we are getting an RID to see if we can sharpen up the peaks and improve separation.
My Question: I found this article (Journal of Chromatography A, 1101 (2006) 115–121) where boric acid is added to the mobile phase at a basic pH to complex to the sugar and enhance the separation while using an Aminex HPX-87K column. I was thinking of trying phenylboronic acid to try to gain the separation and perhaps get a UV signal. I would continue to use my Shodex column. Would my phenylboronic acid destroy my column by bonding to the amines? Is this a stable derivative? Any other suggestions to try are welcome.
Thanks
Background: I am working on a Glucose and Fructose derivative separation. We purchased a Shodex Asahipak NH2P-50 based on a few papers that use the column. We are getting some separation, but it is not baseline resolved (MS detection). I noted in the Shodex literature that their Pb+ column may be better suited. Our current setup is with an LC-DAD-MS and we are getting an RID to see if we can sharpen up the peaks and improve separation.
My Question: I found this article (Journal of Chromatography A, 1101 (2006) 115–121) where boric acid is added to the mobile phase at a basic pH to complex to the sugar and enhance the separation while using an Aminex HPX-87K column. I was thinking of trying phenylboronic acid to try to gain the separation and perhaps get a UV signal. I would continue to use my Shodex column. Would my phenylboronic acid destroy my column by bonding to the amines? Is this a stable derivative? Any other suggestions to try are welcome.
Thanks
