Chromatography Forum

I was hoping someone could help me with the problem of creating a custom field in empower 2 that will allow quantitation of area response in order to calculation % Amount for samples containing a internal standard. I pretty sure I have to set up the x-axis as amount and y-axis value as area response between the two compounds I'm trying to quantify, however I don't know how to enter a custom field for the y-axis that does the area response for standards....any help would be appreciate or if someone has a way of doing it another way I'd appreciate the help.

Hi,

You assign a peak as internal standard to another peak in your processing method, so no extra custom field needed?

Ace

Thanks Aceto_81, however I do realize how to do that, the problem is that I have several methods that want the %Amount or % Label claim found using response ratios. When you click the internal standard it does make one to the other however empower 2 still calculates the % amount using the x-axis amount vs y-axis area. How do I turn this regular area into response ratio...