Residual DMSO and n-butanol determination

[eddie]

Any advices how to determine these residual solvents by liquid/headspace injection?

My molecule has large molecular weight and probably will not elute from column in liquid injection mode.

Is headspace method suitable for DMSO determination?

Thank you in advance

[sg]

what is the solubility of your sample?

[eddie]

Sample soluble in methanol and partially soluble in water

[sg]

I assume it also disolves in dimethylacetamide. If so, then use it as dissolving solvent and use the European pharmacopiea method....

[eddie]

In EP method the incubation temerature is 80 C.
Boling point of DMSO is about 190 C.
How it will affect the method sensitivity?

[sg]

DMSO is class 3 solvent whose limit is 0.5%w/w. So it should be ok. Otherwise can increase the incubation temperature.