Baseline Subtraction using Varian Star Workstation
Posted: Sun Sep 07, 2014 9:53 pm
Hi everyone. I've been working at 225nm wavelength using a methanol gradient, and quite obviously I've been getting serious baseline drift. I want to correct for it, but I'm having a bit of difficulty processing the data files, so...
How do you subtract a baseline run on Star workstation? I ran a blank and labeled it as a "baseline" run on the samplelist. On the method, in the integration parameters, I selected "subtract baseline". So my next question is how to view the results? In the interactive graphics, I open the method, then I open the "baseline" file and my analysis file. When I click "view results" for the analysis run I don't see the subtracted baseline? Do I have to re-integrate? Any help would be appreciated, thank you!
Also, any help on how to use the "Preview Baseline Subtraction" during an actual run would also be really helpful, thanks.
How do you subtract a baseline run on Star workstation? I ran a blank and labeled it as a "baseline" run on the samplelist. On the method, in the integration parameters, I selected "subtract baseline". So my next question is how to view the results? In the interactive graphics, I open the method, then I open the "baseline" file and my analysis file. When I click "view results" for the analysis run I don't see the subtracted baseline? Do I have to re-integrate? Any help would be appreciated, thank you!
Also, any help on how to use the "Preview Baseline Subtraction" during an actual run would also be really helpful, thanks.