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Use for a C1 Column
Posted: Fri Sep 05, 2014 12:37 pm
by algae man
I stumbled across a box of columns in my lab. I was initially excited since the column that had been fouled fairly heavily by the previous user. One of the columns was a Develosil C1. I didn't realize they made that configuration. What kind of use would a C1 see? I'm assuming very low molecular weight compounds but can't think of one that would use such a specific column.
Re: Use for a C1 Column
Posted: Fri Sep 05, 2014 2:49 pm
by tom jupille
What kind of use would a C1 see?
Not much. Possibly very hydrophobic compounds that would be too strongly retained on a longer-chain packing. Perhaps proteins, but the pore size is listed as 140 A ehich is a bit tight for protein work.
Re: Use for a C1 Column
Posted: Mon Sep 08, 2014 5:23 am
by Gerhard Kratz
That is from the Nomura Chemical website:
Separation and retention which can not be predicted from conventional column C1 by the special end capping and the height of the surface area achieved. It corresponds to the analysis of many basic compounds, vitamins and pharmaceuticals including organic acid.
Be happy that you have one in your lab, I'm sure you would never ask to purchase a C1. For compounds with very long retention time I would try the C1 to get it shorter, or for compounds with a tailing. Maybe it will solve some problems later. Keep it.
Good luck.