Oxocarbon separations
Posted: Wed Sep 03, 2014 6:49 pm
Hi, This is hopefully an easy question with an obvious answer. I'm come from a world of alkaloids/C18 columns, and I'm working on HPLC chemistry that is new to me. I'm working on a method to separate inositol, rhodizonate and croconate. I'm struggling to find a good column that works with these guys. I had tried using a C18 (compounds are too hydrophillic) and mixed mode anion column (difficult to work with).
I'd prefer to remain at a neutral pH so the compounds are deprotonated for detection by negative mode by ESI-MS. I have a GC or LC available to me. I didn't see much on the GC. I know that they are somewhat unstable molecules, and I prefer to not derivatize the compounds. Is there a better HPLC packing/separation approach I could try? I have lots of references on synthesis and degradation, but I haven't found much on analysis of these compounds.
Thank you in advance.
I'd prefer to remain at a neutral pH so the compounds are deprotonated for detection by negative mode by ESI-MS. I have a GC or LC available to me. I didn't see much on the GC. I know that they are somewhat unstable molecules, and I prefer to not derivatize the compounds. Is there a better HPLC packing/separation approach I could try? I have lots of references on synthesis and degradation, but I haven't found much on analysis of these compounds.
Thank you in advance.