Dionex DNAPac PA200 issues and questions
Posted: Sat Aug 23, 2014 2:24 am
The protocol I was given and have been following is A: Water B: 2 M NH4OAc ph 7.4.
I thought I put a chromatagram on my flash but I seemed to have forgot it. I have incredible terrible base drift which goes right up with the gradient. I've tried at 1 and 1.5 mL 0-40% 20-50 mins and have okay resolution of small peaks but my major product never have nice peak shape. Should I be running some mM to mM gradient like the application notes I'm reading?
To see better results do I need to switch to a strong cation like sodium? If so which salt system? I see a lot of NaCl, NaHOCl, NaOCl4, etc?
Should I be buffering my water? i.e. x mM Tris pH 8 ?
Does anyone have any opinion if I want to scale up to IE prep on a DNAPac prepscale or the DNASWIFT SAX-1S 5X150 mm ?
Thanks
I thought I put a chromatagram on my flash but I seemed to have forgot it. I have incredible terrible base drift which goes right up with the gradient. I've tried at 1 and 1.5 mL 0-40% 20-50 mins and have okay resolution of small peaks but my major product never have nice peak shape. Should I be running some mM to mM gradient like the application notes I'm reading?
To see better results do I need to switch to a strong cation like sodium? If so which salt system? I see a lot of NaCl, NaHOCl, NaOCl4, etc?
Should I be buffering my water? i.e. x mM Tris pH 8 ?
Does anyone have any opinion if I want to scale up to IE prep on a DNAPac prepscale or the DNASWIFT SAX-1S 5X150 mm ?
Thanks