trimethylamine in Fish

[willemdm]

hi,

currently we want to analyse trimethylamine (TMA) in fish together with other biogenic amines (histamine, cadaverine,...).

i think the best way to analyse them all together would be:

- a pre-column derivatization
- chromatographic analysis with HPLC/UV

the biogenic amines can be derivatised in a large number of ways.

but how do i get TMA in a HPLC/UV suitable form?

grtz

[Markus Laeubli, Metrohm]

If you use cation chromatography with non-suppressed conductivity detection no derivatization is required.

Read the respective article and download the Metrohm Application Note.

[willemdm]

hi,

i had seen the article already, but currently, we don't have the hardware to perform cation exchange chromatography.

therefore we wanted to develop a HPLC method...

grtz

[Gerhard Kratz]

ISRN Biotechnology
Volume 2013 (2013), Article ID 453910, 5 pages
http://dx.doi.org/10.5402/2013/453910
Research Article
A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
Wei Wang,1 Nan Wang,2 Yu Zhang,1 Zheng Cai,1 Qihe Chen,3 and Guoqing He3
1Institute of Quality Standards for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
2College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China
3Departmentof Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China
Received 12 May 2012; Accepted 24 July 2012
Academic Editors: H. S. Garcia and J. Jia
Copyright © 2013 Wei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Maybe this article will help you to a good starting point.
Good luck