convert capillary 1100 hplc to higher flow for porous shell
Posted: Wed Aug 06, 2014 10:18 pm
Hi
I have a capillary 1100 hplc pump which i'd like to work at higher flow rates- say 200-500ul/min using porosshell or halo etc type columns. I wonder what you would suggest about doing this conversion- will i have to replace the microrotor seal and microrotor with a conventional one, replace all peeksil fittings with metal tubing,work in normal flow modeinstead of capillary, utilise a column oven at elevated temps to reduce vbackpressure?
I'dreally appreciate your ideas- there are examples for conventional flow 1100 conversions but none for capillary flow 1100 systems
I have a capillary 1100 hplc pump which i'd like to work at higher flow rates- say 200-500ul/min using porosshell or halo etc type columns. I wonder what you would suggest about doing this conversion- will i have to replace the microrotor seal and microrotor with a conventional one, replace all peeksil fittings with metal tubing,work in normal flow modeinstead of capillary, utilise a column oven at elevated temps to reduce vbackpressure?
I'dreally appreciate your ideas- there are examples for conventional flow 1100 conversions but none for capillary flow 1100 systems