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- Posts: 1
- Joined: Tue Aug 12, 2014 12:57 pm
I have been working on issues with a used agilent 1100 for a few weeks now.
My latest issue is a a problem with resolution and peak broadening.
I am currently trying to run a Bio-Rad gel filtration sample on a Tosoh TSKgel Bio Assist G3SWxl. My mobile phase is 0.1mol Na2So4 in 0.1mol Phosphate buffer (pH6.77). I have tried various flow rates, detection rates, injection speeds, and sample amounts to no avail.
Please, does anyone have any suggestions to get better resolution??
Thanks!!!!
Matt