Chromatography Forum

Posted: **Sun Sep 04, 2005 12:16 pm**

Any advices how to determine these residual solvents by liquid/headspace injection?

My molecule has large molecular weight and probably will not elute from column in liquid injection mode.

Is headspace method suitable for DMSO determination?

Thank you in advance

Posted: **Mon Sep 05, 2005 1:48 am**

what is the solubility of your sample?

Posted: **Mon Sep 05, 2005 8:18 am**

Sample soluble in methanol and partially soluble in water

Posted: **Tue Sep 06, 2005 2:26 am**

I assume it also disolves in dimethylacetamide. If so, then use it as dissolving solvent and use the European pharmacopiea method....

Posted: **Mon Sep 12, 2005 12:32 pm**

In EP method the incubation temerature is 80 C.
Boling point of DMSO is about 190 C.
How it will affect the method sensitivity?

Posted: **Tue Sep 13, 2005 4:15 am**

DMSO is class 3 solvent whose limit is 0.5%w/w. So it should be ok. Otherwise can increase the incubation temperature.

Chromatography Forum

Residual DMSO and n-butanol determination

Residual DMSO and n-butanol determination