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bacterial identification
Posted: Fri Sep 02, 2005 12:30 pm
by vestelshirley
I understand that fatty acid analysis is a way to identify bacteria.
Are there other ways to do this chromatographically?
Posted: Fri Sep 02, 2005 4:28 pm
by Mark Tracy
Yes indeed. There is a standardized protocol for GC analysis of Fatty Acid Methyl Esters and a reference library of bacterial FAME profiles. Ages ago, Hewlett-Packard sold a fully configured system for that application. Ask in the GC forums and I'm sure someone will point you to a current implementation.
Posted: Sat Sep 03, 2005 5:37 pm
by Victor
There are also ways to identify bacterial cells by capillary electrophoresis, although this is not a routine method like the GC procedure Mark described, and CE is not a chromatographic method.....however, if you interested the name to search for is Dan Armstrong who has published a fair bit on this procedure in Analytical Chemistry and other journals.