Chromatography Forum

Hi, All,

I do not know GC.

I f I had sample to analysis, some one directly used each peak area in total peak area to calculate percentage of every fatty acid in total fat. Is this right?

I think That different fatty acid, short or long chain, might give different response factor, and might need to calibrate every fatty acid for calculate their content. Please put your comments and suggestionss, and let me ask them redo the samples correctly. Thank you!

my point of view, there is no right or not right for what you said. someone directly uses area normalization (%area, without response factor) is the easiest way to analyze the content. What you want to do is also correct to know the exact value... so it is fully dependend on your requirement.....

I want true weight content or percentage in a lipid sample.

I consider it is no any meaning for using peak area calculation, if fatty acids response differently. Then, It requires to calibrate fatty acids one by one with own standard.

Fatty acids will have very similar response factors, so using area percent will not be terribly inaccurate. Using standards is best, but I may be difficult to get standards for all fatty acids in a complex sample.

Are you talking about FID? The response is proportional to the number of carbon atoms. Unless you just want a rough estimate I don´t see how one can neglect that. Aren´t there some standard methods for total fat? I would think that´s a bit difficult via FA?