-
- Posts: 175
- Joined: Thu Jul 26, 2007 2:13 pm
I can supply more information if needed, but really the question simplifies to this. Thanks in advance.
Advertisement
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
Considering any liquid chromatography mode as a competition among all the mobile phase components with the analyte for the same stationary phase sites, then a change in peak shape is definitely possible. Depending on which mobile phase additive has weaker adsorption as compared to a given analyte on the stationary phase, one can expect tailed peaks for analytes which interact strongly with the stationary phase. Note that ACN or H2O will also compete, along with HCOOH or H3PO4, with the analyte on the stationary phase adsorption sites.What could the possible causes for an increase in USP tailing from 1.6 to 2.2 just by changing the mobile phases from 0.1% HCOOH in H2O (A) 0.1% HCOOH in ACN (B) to 0.1% H3PO4 in H2O (A) 0.1% H3PO4 in ACN (B)?
I can supply more information if needed, but really the question simplifies to this. Thanks in advance.
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.