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Unknown peak from ammonium sulfate at 260 wavelength

http://www.chromforum.org/viewtopic.php?t=25109

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Unknown peak from ammonium sulfate at 260 wavelength

Posted: Thu Jul 10, 2014 7:31 pm
by hahe0104
I have a unknown peak that absorbs at 260 and is being caused by ammonium sulfate. I am using ACS reagent grade, assay greater then 99%, I am seeing it in both J.T Baker and Sigma ammonium sulfate. Has anyone had this problem before? and does anyone know what could possibly be in the ammonium sulfate or reacting with it to cause this absorption? I am using 10 mM potassium phosphate buffer as my mobile phase pH 3. This peak just so happens to have the exact same retention time as my peak of interest. Any suggestions would help.
Thanks

Re: Unknown peak from ammonium sulfate at 260 wavelength

Posted: Thu Jul 10, 2014 11:47 pm
by tom jupille
Gradient or isocratic?

Re: Unknown peak from ammonium sulfate at 260 wavelength

Posted: Fri Jul 11, 2014 8:33 am
by Gerhard Kratz
Did you run a UV spectrum on a UV photometer of your Ammonium sulfate and your potassium phosphate buffer?
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