Chromatography Forum

I am brand new to the TotalChrom computer system and have a method that is already setup. I ran a set of standards last night on a headspace GC. The standards are set up to run two times wit the first standard does a Replace and the second standard of the same concentration does an Average. The calibration curve has three points. The calibration sequence is set as
1 Blank
2 0.1 standard Replace
3 0.2 standard Replace
4 0.3 standard Replace
5 0.1 standard average
6 0.2 standard average
7 0.3 standard average
The rest of the sequence is standard checks.

After the sequence was run, I figured out how to get a report and at the bottom of each page I have: “Autocalibration Report: Updating Method c:\tc\method\062414\bac B.mth Error – no calibration was performed as no level was specified for the calibration.”

What am I supposed to set as a level? When I go under Method, Components, Calibration the three standards are in the little area that looks like an spreadsheet and the levels are set as B1, B2 and B3. What else do I need to look at?

Here's the calibration component for the method:


Someone else made the method and she is no longer here to ask her about it. And I am learning on my own how to do this.

Thank you.

Christy

When you are processing the standards you are refering to them as B1, B2 and B3 not 0.1 standard etc?
Those are text identifiers so that TotalChrom knows which level is which.