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I think I have no idea what i'm doing.

Posted: Tue Jun 24, 2014 6:23 pm
by yoyooligo
I was shown basics and over time have come close to getting clear separation of my target. I have found by my analytical machine that what I thought should be a clean fraction from my prep was not. Injecting water samples has eluted peaks which I thought my "built in cleaning" method would take care of.

They're both manual injectors and while I was never told details about when to close them back up I've rationalized that a higher %B than initial conditions shouldn't be allowed to enter. I only provide this as an example because I pretty much need an overhaul in technique which I am not getting from my peers. I would like to post and talk about my chromatagrams but I feel there is a lot of noobie stuff I need to go over before hand.

Where do I go/look? I've done my basic rounds of websites like the UCDavis ChemWiki but I'm still left wanting.

Re: I think I have no idea what i'm doing.

Posted: Tue Jun 24, 2014 6:30 pm
by skunked_once
Congratulations! You have taken the correct step in asking for help. From your description, I assume that you are working with HPLC. If so, CHROMacademy has some useful information.

http://www.chromacademy.com/hplc-training.html

Please post more details about your analysis for future questions and we will be glad to help.

Re: I think I have no idea what i'm doing.

Posted: Tue Jun 24, 2014 6:47 pm
by yoyooligo
That resource looks extremely useful and from the descriptions on the direct page linked, looks to have the answers to many of my questions. I may have happened upon this site before, much of it is paid for content? How far can I get without paying?

Re: I think I have no idea what i'm doing.

Posted: Tue Jun 24, 2014 8:44 pm
by skunked_once
I may have happened upon this site before, much of it is paid for content? How far can I get without paying?
Check the "Webcasts and Tutorials" box then click on the topics you are interested in. Several of them have some free content to get you started.

Re: I think I have no idea what i'm doing.

Posted: Wed Jun 25, 2014 12:44 am
by yoyooligo
Made an account, minimal free content. I appreciate the resource but I can't afford the content.

Thank you for.

Re: I think I have no idea what i'm doing.

Posted: Wed Jun 25, 2014 11:32 am
by lmh
If you have an academic e-mail account, you can get the vast majority of CHROMacademy for free, if you ask. If not, you have to pay.

Re: I think I have no idea what i'm doing.

Posted: Wed Jun 25, 2014 1:17 pm
by yoyooligo
I do, and I signed up with it? Do I need to contact someone?

Re: I think I have no idea what i'm doing.

Posted: Wed Jun 25, 2014 7:00 pm
by yoyooligo
Update, sent them an email. Got bumped to premium.