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pH Gradient Mobile phases on a UPLC column

http://www.chromforum.org/viewtopic.php?t=24958

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pH Gradient Mobile phases on a UPLC column

Posted: Sat Jun 14, 2014 11:14 am
by ajey
I am getting peak splitting in all peaks in a following Mobile phase on a UPLC column.: 2.1 x 100mm, 1.7µm

M.P A.: 0.1%OPA pH to 3.0 adjusted with TEA &
M.P B.: (1ml OPA in a200ml Water then added 800ml ACN )pH is around 2.2).

Is it due to different pH mobie phases??

Re: pH Gradient Mobile phases on a UPLC column

Posted: Sat Jun 14, 2014 12:31 pm
by Hollow
Sounds more like a void volume somewhere, as there are all peaks affected.

Re-check/reconnect the installation of the column.

Or the inlet frit of the column may be partially clogged.
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