chiral separation

[jiangds06]

which column or columns are the best columns to cover the biggest chemical space.

[Gerhard Kratz]

This question is not possible to be answered.
I would recommend to use column selection guides from different manufacturers.
And for sure look into the application books from different manufacturers.
There are so many different columns on the market based on different surface chemistries.
I always failed when I tried to select a chiral column for a specific application.
For me it was best to contact 2 or 3 technical support teams from different manufacturers.
At least 3, most times more, suggestions for columns I got. Good luck.

[AA]

If you were only going to buy one, then an AD-H is probably the best bet. We see close to 70% success rate with this column (that also means a 30% failure rate, and success doesn't necessarily mean baseline resolved either). As was hinted in the previous post, you are always better off in chiral to have a selection of 3 or 4 chemistries to maximize your hit rate.

[M Farooq]

which column or columns are the best columns to cover the biggest chemical space.

The question is what class of analytes do you wish to separate?

There are plenty of cyclodextrin and cyclofructan based chiral columns which can separate 100s of chiral compounds. They are compatible with normal phase, reverse phase and under certain circumstances HILIC mode.