Automate my manual events integration in Chemstation B.03.01

[rwilson]

Hello all.

I would like to automate my manual events integration On a 1200 LC using ChemStation B03.01. Let me explain further.

We are a food ingredients R&D lab that analyze a lot of proteins and hydrolysates by Gel permeation SEC. We then need to integrate the chromatogram based on time and not by peaks because we need to know the % within a certain molecular weight range. We run proteins and peptides of know MW on the column and "calibrate" the column. In the past with our 1050 we would bring up a chromatogram in Data analysis, delete all of the peaks, manually redraw the baseline, and manually split the peaks at specific times correlating to the MW we are interested in. We would then click "Copy Manual Events to Method" and make sure the Manual Events box is checked in the events table.

It seems to me that we should be able to do this in the timed events table. I can get a good baseline drawn, and have entered "split peaks" at the times we want. However, the resulting chromatogram does not integrate according to these times. I entered split peaks at 5.968, 6.591, 7.214, 8.036, 8.659, 9.282, & 10.727 minutes. ChemStation then integrates at 6.522, 7.143, 7.765, 8.386, 9.007, 9.628, 9.628 (yes twice), & 10.871 minutes.

Does anyone have any idea how I can automate this integration, it would be so much easier that doing it manually.

Thanks.

[asm]

Hello,

take a look there please:

http://www.chem.agilent.com/Library/Sup ... a10437.pdf

Is it possible, that the "Area Sum On/Off" is what you're looking for?

[rwilson]

ASM.

Thanks for the reply. That's good information, but I don't care about peaks on these chromatograms and this document states that depending on the time the command is set to happen relative to the peak apex determines if the entire peak is integrated or not.

What about turning the integration on and off to bracket each range?

[asm]

Turning the Integration on and of won't help, then he also doesn't integrate the whole area, just single peaks.

But a combination should work.

I just tested a little bit:



With Split Peak and Area Sum it seems to work. (If this is what you want.)

But i think as soon as you have a shift in retentiontime, this won't work anymore and you have to rework the integration events again.

[rwilson]

Let me see if I understand what is going on. We split the peaks at the RT's we want then do area sums between the split peak events. Correct?

As to your comment about RT shift we are aware of this and run the "calibration" samples periodically. Even so, it is much easier to re-enter the RT's in the events table rather than expanding each chromatogram and graphically splitting the peak.

Thanks for your help. If you have any more ideas please post them. I will this on our samples and reply with what I get.

[rwilson]

Hallelujah. We tried it on our samples and it worked. This is going to be a lot easier rather doing this graphically.

Thanks for your help.

[asm]

Glad to hear that it works.