Chromatography Forum

Hello everyone,

I just subscribed to this forum, I didn't know it existed before I did some Google search for my problem... I read all about integration event in the chemstation manual and on this forum (plus a few websites). But I still can't find my solution.

So, I'm a bio-engineering student and I'm doing my thesis to end my degree. Within this work, I have to set new GC methods for new enzyme substrates. I'm working with an enzyme and some variants and I have to define its enantioselectivity etc... To help me I have a GC from Agilent and a PC with Chemstation. I used this software before but not as in depth as I need to use it now.
So I created a new method and set the parameters for my separation (I work on a Cyclosil B column from Agilent). My problem lies in the interpretation of the chromatogram...
I saw that some parameters in "integration event" may be changed. I tried with a method used for another standard and it did not gave me something useful... So I was thinking about changing these values.. But how ! I couldn't find neither on internet or in the manual the way to establish those values for my specific substrate... Maybe a calibration ?

Please tell me if you need more precisions about something.

Thank you for reading and I hope that someone can help me.


Regards

slnx

I would suggest the Auto-Integrate funktion.


Then turn off the integration of your solvent area (add a line with "integration 0.000 off" and a second with "integration [time it should start integration in the chromatogram] on") and play a little bit with the settings "area reject", "hight reject" and "peak width".

Wonderful ! It worked ! Thank you

Now I'm figuring another annoying issue...

The report was generated with the initial integration events (that wasn't adjusted by me). Now that I've changed them, I'd like to export my new report. But I can't find how to do this... (there's no save button as always... and print button do not work at all)
Any idea ?

Post which verion of ChemStation you have. I liked the older "non-enhanced" version of Integration Events better.

Anyway, the Integration Events get saved as part of the Method or Sequence Method, depending on which version you have. Then you would need to Reprocess the data so those new changed parameters will be used.