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Hemoglobin method

Posted: Fri May 09, 2014 5:33 pm
by resolve
I'm assuming that RP or CEX would be the modes of choice for hemoglobin resolution and quantitation.
However, has anyone had any experience or knowledge of being able to resolve the apo (-Fe) from the holoprotein (+Fe).

Thanks in adavnce.

Re: Hemoglobin method

Posted: Sat May 10, 2014 5:28 pm
by Andy Alpert
CEX can effect more difficult separations than this; for example, separation of hemoglobin from hemiglobin.