How to program Empower to make autosample to mix/inj?

[tpmun]

Hello there,

We are trying to run a new method which requires the autosampler to mix 15
ul of common reagent with 15 ul of sample for 1 min before inject this mixture
into the HPLC column.
Would you be able to give us any suggestion on how to program Empower 1 to
do it?

Thank you.

[DR]

What model LC are you using??

[aceto_81]

If you are using an Alliance, you can do this with autoadditions.


Ace

[tpmun]

Thank you for your messages.

We have Waters 717plus Autosampler, Waters 600E Multisolvent Delivery System and Shimadzu RF-535 Fluorescence Monitor. They are controlled by Empower 1 software.

Recently, we be able to program the autosample to mix reagents and then inject the mixture. Unfortunately, we have no idea how to make the Empower controls the autosampler to do that trick.